NULL EFFECT OF TEST ARTICLES AFTER INJECTION OF RATS WITH MYCOBACTERIUM BUTYRICUM

[Contract Research Organization]

Background:

Injections of Mycobacterium butyricum into rats results in edema of the paw. Typically, after such a challenge, paws will increasingly swell to a given maximum, after which the paws plateau in size, and remain stable at that size.

 

Study Design:

Rats (n=10/group) were fed control or immune egg throughout the length of the trial. Day 50 of feeding, M. butyricum was injected into rodents and paw measurements taken on days 69. 72, 76, 83, and 87, and compared to vehicle control. Indomethacin was used as the "positive" control to inhibit edema.

 

Results:

As expected, indomethacin treatment resulted in significant reduction of edema after injections of Mycobacterium butyricum. There were no such observable differences in rat paw edema of animals fed immune egg as compared to control egg.